Electrochemical control of the enzymatic polymerization of PEG hydrogels: formation of spatially controlled biological microenvironments

Adv Healthc Mater. 2014 Apr;3(4):508-14. doi: 10.1002/adhm.201300479. Epub 2013 Oct 30.

Abstract

Control of pH gradient profile at the electrode-electrolyte interfaces allows the control of the enzymatic PEG-hydrogel polymerization. By tuning the solution pH, buffer capacity, and the applied current, the extent of the local inhibition and confinement of the Factor XIII-mediated polymerization of PEG are controlled. This technology opens new perspectives for the production of 3D-structured biological microenvironments.

Keywords: PEG-hydrogels; electrochemistry; microenvironment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / metabolism
  • Cell Engineering / methods*
  • Cell Line
  • Cellular Microenvironment
  • Dimethylpolysiloxanes / chemistry
  • Electrochemical Techniques / methods*
  • Humans
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
  • Hydrogel, Polyethylene Glycol Dimethacrylate / metabolism
  • Hydrogen-Ion Concentration
  • Polyethylene Glycols / chemistry*
  • Polyethylene Glycols / metabolism
  • Polymerization

Substances

  • Biocompatible Materials
  • Dimethylpolysiloxanes
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Polyethylene Glycols
  • baysilon