A regulatory framework for shoot stem cell control integrating metabolic, transcriptional, and phytohormone signals

Dev Cell. 2014 Feb 24;28(4):438-49. doi: 10.1016/j.devcel.2014.01.013.

Abstract

Plants continuously maintain pluripotent stem cells embedded in specialized tissues called meristems, which drive long-term growth and organogenesis. Stem cell fate in the shoot apical meristem (SAM) is controlled by the homeodomain transcription factor WUSCHEL (WUS) expressed in the niche adjacent to the stem cells. Here, we demonstrate that the bHLH transcription factor HECATE1 (HEC1) is a target of WUS and that it contributes to SAM function by promoting stem cell proliferation, while antagonizing niche cell activity. HEC1 represses the stem cell regulators WUS and CLAVATA3 (CLV3) and, like WUS, controls genes with functions in metabolism and hormone signaling. Among the targets shared by HEC1 and WUS are phytohormone response regulators, which we show to act as mobile signals in a universal feedback system. Thus, our work sheds light on the mechanisms guiding meristem function and suggests that the underlying regulatory system is far more complex than previously anticipated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • Cytokinins / genetics
  • Cytokinins / metabolism
  • Gene Expression Regulation, Plant / genetics
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • Meristem / genetics
  • Meristem / metabolism
  • Plant Growth Regulators / genetics
  • Plant Growth Regulators / metabolism*
  • Plant Shoots / genetics
  • Plant Shoots / metabolism
  • Plant Stems / genetics*
  • Plant Stems / metabolism
  • Signal Transduction / genetics*
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic*

Substances

  • Arabidopsis Proteins
  • CLV3 protein, Arabidopsis
  • Cytokinins
  • HEC1 protein, Arabidopsis thaliana
  • Homeodomain Proteins
  • Plant Growth Regulators
  • Transcription Factors
  • WUSCHEL protein, Arabidopsis