RNAi Screening in Drosophila Cells and in Vivo

Methods. 2014 Jun 15;68(1):82-8. doi: 10.1016/j.ymeth.2014.02.018. Epub 2014 Feb 24.

Abstract

Here, I discuss how RNAi screening can be used effectively to uncover gene function. Specifically, I discuss the types of high-throughput assays that can be done in Drosophila cells and in vivo, RNAi reagent design and available reagent collections, automated screen pipelines, analysis of screen results, and approaches to RNAi results verification.

Keywords: Cell-based assays; Drosophila; Functional genomics; High-throughput screening; In vivo assays; RNAi.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Developmental Biology / methods*
  • Drosophila
  • Genomics
  • High-Throughput Screening Assays / methods*
  • RNA Interference*