Here, I discuss how RNAi screening can be used effectively to uncover gene function. Specifically, I discuss the types of high-throughput assays that can be done in Drosophila cells and in vivo, RNAi reagent design and available reagent collections, automated screen pipelines, analysis of screen results, and approaches to RNAi results verification.
Keywords: Cell-based assays; Drosophila; Functional genomics; High-throughput screening; In vivo assays; RNAi.
Copyright © 2014. Published by Elsevier Inc.