The orphan nuclear receptor SF-1 is involved in the effect of PCBs, DDT, and DDE on the secretion of steroid hormones and oxytocin from bovine luteal cells during the estrous cycle in vitro

J Mlynarczuk; M H Wrobel; J Kotwica

doi:10.1016/j.theriogenology.2014.01.016

The orphan nuclear receptor SF-1 is involved in the effect of PCBs, DDT, and DDE on the secretion of steroid hormones and oxytocin from bovine luteal cells during the estrous cycle in vitro

Theriogenology. 2014 Apr 15;81(7):877-86. doi: 10.1016/j.theriogenology.2014.01.016. Epub 2014 Jan 30.

Authors

J Mlynarczuk¹, M H Wrobel¹, J Kotwica²

Affiliations

¹ Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
² Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland. Electronic address: janko@pan.olsztyn.pl.

PMID: 24576709
DOI: 10.1016/j.theriogenology.2014.01.016

Abstract

The orphan receptor steroidogenic factor-1 (SF-1) is involved in the regulation of ovarian steroidogenesis in cows. It is hypothesized that estrogen-like chlorinated compounds might affect SF-1, and thus impair the function of the ovary. Bovine luteal cells from the estrous cycle (Days: 1-5, 6-10, 11-15, and 16-19) were treated for 50 hours with DDT, 1,1-dichloro-2,2-bis(4-chlorophenyl)ethene, 3,3'4,4'-tetrachlorobiphenyl or 2'2'4,4',5,5'-hexachlorobiphenyl (each at a dose of 10 ng/mL). Luteal cells were also treated with 4-(heptyloxy)phenol (1 × 10(-7) M), an SF-1 agonist, and F0160 (1 × 10(-6) M), an SF-1 blocker, jointly or separately. The secretion of progesterone and oxytocin and the expression of oxytocin precursor (NP-I/OT) messenger RNA were increased (P < 0.05) by all studied xenobiotics and 4-(heptyloxy)phenol, although they were inhibited (P < 0.05) by F0160. However, the xenobiotics did not affect (P > 0.05) SF-1 messenger RNA expression. In summary, SF-1 is involved in the adverse effect of chlorinated xenobiotics on the regulation of the bovine CL.

Keywords: Chlorinated xenobiotics; Cow; Luteal cell; Oxytocin; Progesterone; Steroidogenic factor-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle*
Cells, Cultured
DDT / toxicity
Dichlorodiphenyl Dichloroethylene / toxicity
Environmental Pollutants / toxicity*
Estrous Cycle / physiology*
Female
Gene Expression Regulation / physiology
Luteal Cells / metabolism*
Oxytocin / metabolism
Polychlorinated Biphenyls / toxicity
Steroidogenic Factor 1 / metabolism*
Steroids / metabolism

Substances

Environmental Pollutants
Steroidogenic Factor 1
Steroids
Dichlorodiphenyl Dichloroethylene
Oxytocin
DDT
Polychlorinated Biphenyls