Precisely and accurately localizing single emitters in fluorescence microscopy

doi:10.1038/nmeth.2843

Review

. 2014 Mar;11(3):253-66.

doi: 10.1038/nmeth.2843.

Precisely and accurately localizing single emitters in fluorescence microscopy

Hendrik Deschout¹, Francesca Cella Zanacchi², Michael Mlodzianoski³, Alberto Diaspro², Joerg Bewersdorf⁴, Samuel T Hess³, Kevin Braeckmans¹

Affiliations

¹ 1] Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium. [2] Center for Nano- and Biophotonics, Ghent University, Ghent, Belgium.
² 1] Department of Nanophysics, Italian Institute of Technology, Genoa, Italy. [2] Department of Physics, University of Genoa, Genoa, Italy.
³ Department of Physics and Astronomy, University of Maine, Orono, Maine, USA.
⁴ 1] Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA. [2] Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA.

PMID: 24577276
DOI: 10.1038/nmeth.2843

Review

Precisely and accurately localizing single emitters in fluorescence microscopy

Hendrik Deschout et al. Nat Methods. 2014 Mar.

. 2014 Mar;11(3):253-66.

doi: 10.1038/nmeth.2843.

Authors

Hendrik Deschout¹, Francesca Cella Zanacchi², Michael Mlodzianoski³, Alberto Diaspro², Joerg Bewersdorf⁴, Samuel T Hess³, Kevin Braeckmans¹

Affiliations

¹ 1] Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium. [2] Center for Nano- and Biophotonics, Ghent University, Ghent, Belgium.
² 1] Department of Nanophysics, Italian Institute of Technology, Genoa, Italy. [2] Department of Physics, University of Genoa, Genoa, Italy.
³ Department of Physics and Astronomy, University of Maine, Orono, Maine, USA.
⁴ 1] Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA. [2] Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA.

PMID: 24577276
DOI: 10.1038/nmeth.2843

Abstract

Methods based on single-molecule localization and photophysics have brought nanoscale imaging with visible light into reach. This has enabled single-particle tracking applications for studying the dynamics of molecules and nanoparticles and contributed to the recent revolution in super-resolution localization microscopy techniques. Crucial to the optimization of such methods are the precision and accuracy with which single fluorophores and nanoparticles can be localized. We present a lucid synthesis of the developments on this localization precision and accuracy and their practical implications in order to guide the increasing number of researchers using single-particle tracking and super-resolution localization microscopy.

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Comment in

The elegance of localization microscopy and the promise it holds.
Mondal PP. Mondal PP. Microsc Res Tech. 2015 Jan;78(1):11-2. doi: 10.1002/jemt.22442. Epub 2014 Oct 7. Microsc Res Tech. 2015. PMID: 25291709 No abstract available.

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References

1. Nano Lett. 2009 Jul;9(7):2676-82 - PubMed
1. Opt Lett. 2011 Jan 15;36(2):202-4 - PubMed
1. Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19087-92 - PubMed
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[1] Nano Lett. 2009 Jul;9(7):2676-82 - PubMed

[2] Nano Lett. 2009 Jul;9(7):2676-82 - PubMed

[3] Opt Lett. 2011 Jan 15;36(2):202-4 - PubMed

[4] Opt Lett. 2011 Jan 15;36(2):202-4 - PubMed

[5] Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19087-92 - PubMed

[6] Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19087-92 - PubMed

[7] Nat Methods. 2014 Mar;11(3):267-79 - PubMed

[8] Nat Methods. 2014 Mar;11(3):267-79 - PubMed

[9] Nat Methods. 2012 Oct;9(10):938-9 - PubMed

[10] Nat Methods. 2012 Oct;9(10):938-9 - PubMed

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Precisely and accurately localizing single emitters in fluorescence microscopy

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Precisely and accurately localizing single emitters in fluorescence microscopy

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