Doxorubicin-induced oxidative stress in rats is efficiently counteracted by dietary anthocyanin differently enriched strawberry (Fragaria × ananassa Duch.)

J Agric Food Chem. 2014 May 7;62(18):3935-43. doi: 10.1021/jf405721d. Epub 2014 Mar 11.


This study investigated the effects of two different strawberry cultivars, Adria and Sveva, against doxorubicin (DOX)-induced toxicity in rats. A controlled dietary intervention was conducted over 16 weeks with four groups: (i) normal diet; (ii) normal diet + DOX injection; (iii) Adria supplementation + DOX injection; and (iv) Sveva supplementation + DOX injection. Sveva presented higher total antioxidant capacity value and phenol and and vitamin C levels than Adria, which in turn presented higher anthocyanin contents. DOX drastically increased lymphocyte DNA damage, liver biomarkers of protein and lipid oxidation, and mitochondrial ROS content and markedly decreased plasma retinol level, liver antioxidant enzymes, and mitochondrial functionality. After 2 months of strawberry supplementation, rats presented a significant reduction of DNA damage and ROS concentration and a significant improvement of oxidative stress biomarkers, antioxidant enzyme activities, and mitochondrial performance. These results suggest that strawberry supplementation can counteract DOX toxicity, confirming the potential health benefit of strawberry in vivo against oxidative stress.

Keywords: DNA damage; anthocyanins; doxorubicin; mitochondrial functionality; oxidative stress; strawberry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthocyanins / metabolism*
  • Chemical and Drug Induced Liver Injury / diet therapy*
  • Chemical and Drug Induced Liver Injury / etiology
  • Chemical and Drug Induced Liver Injury / genetics
  • Chemical and Drug Induced Liver Injury / metabolism
  • DNA Damage / drug effects
  • Doxorubicin / toxicity*
  • Fragaria / metabolism*
  • Humans
  • Liver / drug effects
  • Liver / enzymology
  • Liver / metabolism
  • Male
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Oxidative Stress* / drug effects
  • Rats
  • Rats, Wistar


  • Anthocyanins
  • Doxorubicin