A surface-enhanced Raman scattering method for detection of trace glutathione on the basis of immobilized silver nanoparticles and crystal violet probe

Anal Chim Acta. 2014 Mar 13:816:41-9. doi: 10.1016/j.aca.2014.01.046. Epub 2014 Feb 3.

Abstract

Unsatisfactory sensitivity and stability for molecules with low polarizability is still a problem limiting the practical applications of surface-enhanced Raman scattering (SERS) technique. By preparing immobilized silver nanoparticles (Fe3O4/Ag) through depositing silver on the surface of magnetite particles, a highly sensitive and selective SERS method for the detection of trace glutathione (GSH) was proposed on the basis of a system of Fe3O4/Ag nanoparticles and crystal violet (CV), in which the target GSH competed with the CV probe for the adsorption on the Fe3O4/Ag nanoparticles. Raman insensitive GSH replaced the highly Raman sensitive CV adsorbed on the surface of Fe3O4/Ag particles. This replacement led to a strong decrease of the CV SERS signal, which was used to determine the concentration of GSH. Under optimal conditions, a linear response was established between the intensity decrease of the CV SERS signal and the GSH concentration in the range of 50-700 nmol L(-1) with a detection limit of 40 nmol L(-1). The use of a Fe3O4/Ag substrate provided not only a great SERS enhancement but also a good stability, which guarantees the reproducibility of the proposed method. Its use for the determination of GSH in practical blood samples and cell extract yielded satisfactory results.

Keywords: Crystal violet; Glutathione; Immobilized silver nanoparticles; Replacement.; Surface-enhanced Raman scattering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Ferric Compounds / chemistry
  • Gentian Violet / chemistry*
  • Glutathione / analysis*
  • Metal Nanoparticles / chemistry*
  • Particle Size
  • Silver / chemistry*
  • Spectrum Analysis, Raman
  • Surface Properties

Substances

  • Ferric Compounds
  • ferric oxide
  • Silver
  • Glutathione
  • Gentian Violet