Interplay of acetyltransferase EP300 and the proteasome system in regulating heat shock transcription factor 1

Cell. 2014 Feb 27;156(5):975-85. doi: 10.1016/j.cell.2014.01.055.


When exposed to proteotoxic environmental conditions, mammalian cells activate the cytosolic stress response in order to restore protein homeostasis. A key feature of this response is the heat shock transcription factor 1 (HSF1)-dependent expression of molecular chaperones. Here, we describe the results of an RNA interference screen in HeLa cells to identify modulators of stress response induction and attenuation. The modulator proteins are localized in multiple cellular compartments, with chromatin modifiers and nuclear protein quality control playing a central regulatory role. We find that the acetyltransferase, EP300, controls the cellular level of activatable HSF1. This involves acetylation of HSF1 at multiple lysines not required for function and results in stabilization of HSF1 against proteasomal turnover. Acetylation of functionally critical lysines during stress serves to fine-tune HSF1 activation. Finally, the nuclear proteasome system functions in attenuating the stress response by degrading activated HSF1 in a manner linked with the clearance of misfolded proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • Cell Nucleus / metabolism
  • DNA-Binding Proteins / metabolism*
  • E1A-Associated p300 Protein / metabolism*
  • HEK293 Cells
  • HeLa Cells
  • Heat Shock Transcription Factors
  • Heat-Shock Response
  • Humans
  • Proteasome Endopeptidase Complex / metabolism*
  • Protein Folding
  • Protein Interaction Maps
  • Proteome / analysis
  • Proteome / metabolism
  • Transcription Factors / metabolism*


  • DNA-Binding Proteins
  • HSF1 protein, human
  • Heat Shock Transcription Factors
  • Proteome
  • Transcription Factors
  • E1A-Associated p300 Protein
  • EP300 protein, human
  • Proteasome Endopeptidase Complex