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. 2014 Mar 2.
doi: 10.1007/s11655-013-1534-3. Online ahead of print.

Bioactivity-guided Identification to Delineate the Immunomodulatory Effects of Methanolic Extract of Nigella Sativa Seed on Human Peripheral Blood Mononuclear Cells

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Bioactivity-guided Identification to Delineate the Immunomodulatory Effects of Methanolic Extract of Nigella Sativa Seed on Human Peripheral Blood Mononuclear Cells

Ali A Alshatwi. Chin J Integr Med. .

Abstract

Objective: To study the immunomodulatory effects of Nigella sativa (NS) on human peripheral blood mononuclear cells (PBMCs) on a Phytohemagglutinin (PHA) and a non-PHA stimulated proliferation.

Methods: Cells isolated from human PBMCs which were treated with methanolic extract of NS for 48 h into two separate environments (PHA and non-PHA stimulated). Flow cytometry (for T helper/inducer cells and natural killer cells) and real time-polymerase chain reaction (PCR) assays for a few selected proinflammatory gene expressions were performed.

Results: Extracts from NS had an immunostimulating effect on non-PHA-stimulated proliferation of human PBMCs. In contrast, immunosuppressive activity was observed on PHA-stimulated proliferation of human PBMCs.

Conclusion: This in vitro study revealed the effects of NS plant extract on nonspecifific cellular immune responses.

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