Profiling of autoantibodies in sera of pancreatic cancer patients

Ann Surg Oncol. 2014 Jun;21 Suppl 3:S459-65. doi: 10.1245/s10434-014-3574-0. Epub 2014 Mar 1.

Abstract

Background: Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system.

Methods: We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen® assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated.

Results: Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 %, respectively, and 97, 35, 74, and 88 %, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics.

Conclusion: Several autoantibodies, including CIB1, are potential biomarkers for PC.

Publication types

  • Comparative Study
  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Aged
  • Antigens, Neoplasm / immunology*
  • Autoantibodies / blood*
  • Biomarkers, Tumor / blood*
  • Calcium-Binding Proteins / blood*
  • Case-Control Studies
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Follow-Up Studies
  • Humans
  • Male
  • Middle Aged
  • Pancreatic Neoplasms / blood*
  • Pancreatic Neoplasms / diagnosis
  • Pancreatic Neoplasms / immunology
  • Prognosis
  • Sensitivity and Specificity

Substances

  • Antigens, Neoplasm
  • Autoantibodies
  • Biomarkers, Tumor
  • CIB1 protein, human
  • Calcium-Binding Proteins