Automated cell-free protein production methods for structural studies

Methods Mol Biol. 2014;1140:117-35. doi: 10.1007/978-1-4939-0354-2_9.

Abstract

In contrast to cell-based protein expression, cell-free production is highly consistent, scalable, and amenable to automation. Robots can handle many samples and perform repetitive procedures that are otherwise prone to human error. Here is described commercially available robotics for a wheat germ cell-free system with emphasis on practical applications for structural and functional studies. In addition, described is a cell-free method for preparing protein complexes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Automation / methods
  • Cell-Free System*
  • Humans
  • Molecular Biology / methods*
  • Protein Biosynthesis*
  • Protein Conformation
  • Proteomics
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Triticum / genetics

Substances

  • Recombinant Proteins