The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice

Environ Toxicol. 2013 Dec;28(12):681-8. doi: 10.1002/tox.20763. Epub 2011 Aug 31.


This study was aimed at the investigation of the antioxidant effect of pumpkin seed oil against the oxidative stress-inducing potential of aflatoxin. For this purpose, 48 male BALB/c mice were used. Four groups, each comprising 12 mice, were established. Group 1 was maintained as the control group. Group 2 was administered with pumpkin seed oil alone at a dose of 1.5 mL/ (∼1375mg/ Group 3 received aflatoxin (82.45% AFB1 , 10.65% AFB2 , 4.13% AFG1, and 2.77% AFG2 ) alone at a dose of 625 μg/ Finally, group 4 was given both 1.5 mL/ pumpkin seed oil and 625 μg/ aflatoxin. All administrations were oral, performed with the aid of a gastric tube and continued for a period of 21 days. At the end of day 21, the liver, lungs, kidneys, brain, heart, and spleen of the animals were excised, and the extirpated tissues were homogenized appropriately. Malondialdehyde (MDA) levels and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities were determined in tissue homogenates. In conclusion, it was determined that aflatoxin exhibited adverse effects on most of the oxidative stress markers. The administration of pumpkin seed oil diminished aflatoxin-induced adverse effects. In other words, the values of the group, which was administered with both aflatoxin and pumpkin seed oil, were observed to have drawn closer to the values of the control group.

Keywords: aflatoxin; antioxidant effect; mice; pumpkin seed oil.

MeSH terms

  • Aflatoxins / toxicity*
  • Animals
  • Antioxidants / pharmacology*
  • Catalase / metabolism
  • Cucurbita / chemistry*
  • Lipid Peroxidation / drug effects
  • Male
  • Malondialdehyde / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Organ Specificity
  • Oxidation-Reduction
  • Oxidative Stress / drug effects
  • Plant Oils / pharmacology*
  • Seeds / chemistry
  • Superoxide Dismutase / metabolism


  • Aflatoxins
  • Antioxidants
  • Plant Oils
  • Malondialdehyde
  • Catalase
  • Superoxide Dismutase