Molecular detection of hepatitis E virus in rivers in the Philippines

Tian-Cheng Li; Tingting Yang; Tomoyuki Shiota; Sayaka Yoshizaki; Hiromu Yoshida; Mariko Saito; Toshifumi Imagawa; Fidelino F Malbas; Socorro P Lupisan; Hitoshi Oshitani; Takaji Wakita; Koji Ishii

doi:10.4269/ajtmh.13-0562

Molecular detection of hepatitis E virus in rivers in the Philippines

Am J Trop Med Hyg. 2014 Apr;90(4):764-6. doi: 10.4269/ajtmh.13-0562. Epub 2014 Mar 3.

Authors

Tian-Cheng Li¹, Tingting Yang, Tomoyuki Shiota, Sayaka Yoshizaki, Hiromu Yoshida, Mariko Saito, Toshifumi Imagawa, Fidelino F Malbas, Socorro P Lupisan, Hitoshi Oshitani, Takaji Wakita, Koji Ishii

Affiliation

¹ Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-Murayama, Tokyo 208-0011, Japan; Department of Clinical Laboratory, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China; Department of Virology, Tohoku University Graduate School of Medicine, Miyagi 980-8575, Japan; RITM-Tohoku Collaborating Research Center on Emerging and Re-Emerging Infectious Diseases, Filinvest Corporate City, Alabang, Muntinlupa City 1781, the Philippines; Research Institute for Tropical Medicine, Department of Health Compound, FILINVEST Corporate City, Alabang, Muntinlupa City 1781, the Philippines.

Abstract

To understand the hepatitis E virus (HEV)-pollution status in the environment in the Philippines, a total of 12 water samples were collected from rivers in Manila City for detection of HEV RNA. Three of 12 samples were positive for HEV RNA indicating that HEV is circulating in the Philippines. Phylogenetic analysis classified all of the HEV sequences into genotype 3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Hepatitis E virus / classification
Hepatitis E virus / genetics*
Hepatitis E virus / isolation & purification
Philippines
RNA, Viral / analysis*
Reverse Transcriptase Polymerase Chain Reaction
Rivers / virology*

Substances

RNA, Viral