Immunoglobulin heavy chain/light chain pairs (HLC, Hevylite™) assays for diagnosing and monitoring monoclonal gammopathies

Adv Clin Exp Med. 2014 Jan-Feb;23(1):127-33. doi: 10.17219/acem/37036.

Abstract

Immunofixation (IFE) is a standard method for detecting monoclonal immunoglobulins and characterizing its isotype. Recently clonality can also be determined by using immunoglobulin (Ig) heavy chain/light chain immunoassays - HLC, HevyliteTM. HLC separately measures in pairs light chain types of each intact Ig class generating ratios of monoclonal Ig/uninvolved polyclonal Ig concentrations. Studies have shown that HLC and IFE are complementary methods. HLC assays quantify monoclonal proteins and identify monoclonality. It is possible to predict prognosis in multiple myeloma and to monitor response to treatment using HLC ratio. HLC ratio may serve as a parameter for myeloma induced immunoparesis and serve as a new marker for validating remission depth and relapse probabilities.

Publication types

  • Review

MeSH terms

  • Biomarkers
  • Humans
  • Immunoassay
  • Immunoglobulin Heavy Chains / blood*
  • Immunoglobulin Light Chains / blood*
  • Paraproteinemias / diagnosis*
  • Paraproteinemias / immunology
  • Prognosis

Substances

  • Biomarkers
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains