TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella

Elife. 2014 Jan 1;3:e01566. doi: 10.7554/eLife.01566.


Cilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26/DYF13. Using live imaging and biochemical approaches we show that TTC26/DYF13 is an IFT complex B protein in mammalian cells and Chlamydomonas reinhardtii. Knockdown of TTC26/DYF13 in zebrafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motility. Surprisingly, IFT particle assembly and speed were normal in dyf13 mutant flagella, unlike in other IFT complex B mutants. Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant. These results support the concept that different IFT proteins are responsible for different cargo subsets, providing a possible explanation for the complexity of the IFT machinery. DOI:

Keywords: Chlamydomonas; axoneme; dynein; flagella.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Algal Proteins / genetics
  • Algal Proteins / metabolism*
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cell Movement*
  • Chlamydomonas reinhardtii / genetics
  • Chlamydomonas reinhardtii / metabolism*
  • Cilia / metabolism*
  • Embryo, Nonmammalian / metabolism
  • Flagella / metabolism*
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Genotype
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Mice
  • Mutation
  • Phenotype
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Protein Transport
  • Transfection
  • Zebrafish
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism*


  • Algal Proteins
  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Plant Proteins
  • Ttc26 protein, mouse
  • Ttc26 protein, zebrafish
  • Zebrafish Proteins