Comparison of HMB-45 monoclonal antibody and S-100 protein in the immunohistochemical diagnosis of melanoma

Am J Clin Pathol. 1988 Oct;90(4):385-90. doi: 10.1093/ajcp/90.4.385.


The value of HMB-45 mouse monoclonal antibody in the immunohistochemical diagnosis of melanoma was compared with that of two antibodies to S-100 protein. Tissue from 32 (91.4%) of the 35 melanomas studied reacted with HMB-45, however, none of the 98 nonmelanoma tumors stained with this antibody. In contrast, 31 (88.5%) melanomas and 24 (24.4%) of the nonmelanoma neoplasms expressed S-100 protein. These results indicated that HMB-45 monoclonal antibody has a higher specificity for melanoma cells than did S-100 protein and that, because of its reactivity in routinely processed tissues, it may be helpful in surgical pathology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal*
  • Antigen-Antibody Reactions
  • Antigens, Neoplasm
  • Humans
  • Immunohistochemistry* / methods
  • Indicators and Reagents
  • Melanoma / analysis
  • Melanoma / pathology*
  • Melanoma-Specific Antigens
  • Neoplasm Metastasis
  • Neoplasm Proteins / analysis
  • S100 Proteins / analysis*
  • S100 Proteins / immunology
  • Staining and Labeling* / methods


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Indicators and Reagents
  • Melanoma-Specific Antigens
  • Neoplasm Proteins
  • S100 Proteins