Antibody produced against isolated Rh(D) polypeptide reacts with other Rh-related antigens

Blood. 1988 Nov;72(5):1622-6.

Abstract

Rh(D) antigen-containing polypeptide was prepared by immune precipitation of intact cDE/cDE erythrocytes by using a high-titer preparation of polyclonal anti-D. when isolated Rh(D) polypeptide was administered to rabbits, antibody was produced that was unresponsive toward Rh-positive and -negative cells but reacted strongly with the immunogen in enzyme-linked immunosorbent assay-type immunobinding and Western blot immunostaining assays. Rabbit antibody also immunostains isolated Rh(c) polypeptide as well as the Rh antigen-containing components of sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated membrane proteins from Rh(D)-positive (cDE/cDE,CDe/CDe), Rh(D)-negative (cde/cde,Cde/Cde), and -D-/-D- cells. It does not react with any membrane protein from Rh-null regulator type cells, thus indicating a specificity for Rh-related proteins. We have also been able to demonstrate that polyclonal and monoclonal anti-D preparations that do not immunostain isolated Rh(D) polypeptide will react with it in our immunobinding assay.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibody Specificity
  • Antigens / immunology*
  • Blotting, Western
  • Cell Membrane / immunology
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes
  • Humans
  • Membrane Proteins / immunology
  • Molecular Weight
  • Rh-Hr Blood-Group System / immunology*

Substances

  • Antigens
  • Epitopes
  • Membrane Proteins
  • Rh-Hr Blood-Group System
  • Rho(D) antigen