Peripheral monocytes of obese women display increased chemokine receptor expression and migration capacity

J Clin Endocrinol Metab. 2014 Jul;99(7):2500-9. doi: 10.1210/jc.2013-2611. Epub 2014 Feb 25.


Context: The activation of peripheral immune cells and the infiltration of immune cells into adipose tissue in obesity are implicated in the development of type 2 diabetes mellitus.

Objective: The aim of the study was to compare peripheral immune cells from obese and normal-weight women with regard to composition of immune cell subpopulations, surface expression of the chemokine receptors (CCRs) CCR2, CCR3, CCR5, and CXCR3 (chemokine (C-X-C motif) receptor 3) and cell-intrinsic migration capacity.

Design: This was a case-control study.

Setting: The study was conducted at a university clinical study center.

Patients: Obese females and normal-weight females were included for fluorescence-activated cell sorting analysis and migration assays.

Main outcome measures: Peripheral blood mononuclear cells were prepared from fasting blood samples and used for fluorescence-activated cell sorting analysis and migration assays.

Results: An increase in the percentages of CD14(+)CD16(+) monocytes was observed in obese subjects compared with controls. The CCR profile of monocytes differed significantly in the obese state; in particular, CCR2 levels were increased. In addition, a higher chemotactic activity of monocytes from obese subjects was observed in a migration assay, which was associated with both insulin resistance and CCR2 expression.

Conclusion: Our results suggest that the enhanced intrinsic migratory capacity of peripheral monocytes in obese women may be due to the increased CCR expression, further supporting a link between peripheral immune cell dysfunction and obesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Case-Control Studies
  • Chemotaxis, Leukocyte* / genetics
  • Female
  • Gene Expression
  • Humans
  • Ideal Body Weight
  • Monocytes / metabolism*
  • Obesity / blood*
  • Obesity / genetics*
  • Receptors, Chemokine / genetics*
  • Receptors, Chemokine / metabolism
  • Thinness / blood
  • Thinness / genetics
  • Up-Regulation / genetics


  • Receptors, Chemokine