Design of clone-specific probes from genome sequences for rapid PCR-typing of outbreak pathogens

Clin Microbiol Infect. 2014 Nov;20(11):O891-3. doi: 10.1111/1469-0691.12616. Epub 2014 Apr 4.


The genome sequence of one OXA-48-producing Klebsiella pneumoniae belonging to sequence type (ST) 405, and three belonging to ST11, were used to design and test ST-specific PCR assays for typing OXA-48-producing K. pneumoniae. The approach proved to be useful for in-house development of rapid PCR typing assays for local outbreak surveillance.

Keywords: Bacterial genomes; Klebsiella pneumoniae; OXA-48; PCR typing; hospital infection.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques / methods*
  • Disease Outbreaks*
  • Genotyping Techniques / methods*
  • Humans
  • Klebsiella Infections / epidemiology*
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / classification*
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Polymerase Chain Reaction / methods*