Characterization of a polyubiquitin gene from Arabidopsis thaliana

Mol Gen Genet. 1988 Aug;213(2-3):435-43. doi: 10.1007/BF00339613.

Abstract

Ubiquitin DNA sequences were isolated from the higher plant Arabidopsis thaliana L. by screening a lambda-gt11 genomic library with antibodies raised against oat and human ubiquitin. DNA sequence analysis showed that the predicted protein sequence is 100% conserved with that found in oat and barley and differs by only three residues to that found in animals. This gene (UBQ4) encodes a ubiquitin polyprotein with five repeats contiguously linked with no intervening sequences in the coding region and a C-terminal extension of Ser-Phe. Genomic Southern blot analysis showed that ubiquitin sequences comprise a multigene family of approximately 11 members in Arabidopsis. Northern blot analysis identified at least four transcript size classes, which accumulate in sizes ranging from 800 to 1900 bases. A 5'-specific probe for the UBQ4 gene was used to show that after 2 h heat shock stress, the steady state mRNA level decreased significantly in flowers/buds but not in leaves. The UBQ4 transcript accumulates in a differential manner, accumulating to higher levels in germinating tissue, etiolated tissue, and flowers/buds than in mature leaves, roots, or stems.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Heat-Shock Proteins / genetics
  • Molecular Sequence Data
  • Multigene Family
  • Plants / genetics*
  • Plants / metabolism
  • RNA / genetics
  • RNA / metabolism
  • Restriction Mapping
  • Transcription, Genetic
  • Ubiquitins / genetics*

Substances

  • Heat-Shock Proteins
  • Ubiquitins
  • RNA
  • DNA

Associated data

  • GENBANK/X12853