Adenosine-5'-triphosphate-sensitive ion channels in neonatal rat cultured central neurones

Pflugers Arch. 1988 Aug;412(3):297-304. doi: 10.1007/BF00582512.

Abstract

ATP-sensitive channels were observed in isolated inside-out membrane patches from rat cultured central neurones. Two types of ATP-sensitive K+ channels were present in cortical neurones, one which had its open-state probability increased, the other its open-state probability decreased by application of ATP to the cytoplasmic membrane surface. Another, ATP-sensitive channel differing in ion conductance from all previously reported ATP-sensitive channels was also seen in patches from cortical neurones. This channel was nonselective with respect to Na+, K+ and Cl- ions and ATP produced a "flickery" type of block. The non-hydrolysable analogue, AMPPNP, did not mimic ATP and prevented ATP action. Preliminary experiments indicate that similar, but not, identical ATP-sensitive channels exist in cerebellar neurones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / pharmacology*
  • Animals
  • Animals, Newborn / physiology*
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Cerebellum / cytology
  • Cerebellum / metabolism*
  • Cerebellum / ultrastructure
  • Cerebral Cortex / cytology
  • Cerebral Cortex / metabolism*
  • Cerebral Cortex / ultrastructure
  • Ion Channels / drug effects*
  • Neurons / metabolism*
  • Potassium Channels / drug effects
  • Rats

Substances

  • Ion Channels
  • Potassium Channels
  • Adenosine Triphosphate