Characterization of DNA polymerase beta mRNA: cell-cycle and growth response in cultured human cells

Nucleic Acids Res. 1988 Oct 25;16(20):9587-96. doi: 10.1093/nar/16.20.9587.


DNA polymerase beta (beta-polymerase) is a housekeeping enzyme involved in DNA repair in vertebrate cells. We used a cDNA probe to study abundance of beta-polymerase mRNA in cultured human cells. The mRNA level in synchronized HeLa cells, representing different stages of the cell-cycle, varied only slightly. Contact inhibited fibroblasts AG-1522 contained the same level of mRNA as growing cells. The steady-state level of mRNA in fibroblasts is equivalent to 6 molecules per cell. The results indicate that the beta-polymerase transcript is "low abundance" and is neither cell-cycle nor growth phase responsive.

MeSH terms

  • Cell Cycle*
  • Cell Division*
  • Contact Inhibition
  • DNA Polymerase I / isolation & purification
  • DNA Polymerase I / metabolism*
  • Fibroblasts / enzymology
  • Fibroblasts / metabolism
  • Fibroblasts / physiology
  • HeLa Cells / enzymology*
  • HeLa Cells / metabolism
  • HeLa Cells / physiology
  • Humans
  • Interphase
  • Poly A / isolation & purification
  • RNA / isolation & purification
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism*


  • RNA, Messenger
  • Poly A
  • RNA
  • DNA Polymerase I