Calbindin-D28K and the peptidergic neuroendocrine system in rat gut: an immunohistochemical study

Biol Cell. 1988;63(1):67-75.

Abstract

Calbindin-D28K was immunohistochemically localized in myenteric and submucosal plexuses throughout the rat intestine. Calbindin-D28K immunoreactivity was found in about half of myenteric neurons and in more than 90% of submucosal neurons. Calbindin-D28K was also observed in nerve processes running inside ganglia, muscle layers and lamina propria. No correlation could be established between the presence of calbindin-D28K and the distribution of neuropeptides localized in this study (VIP, enkephalin, somatostatin and substance P). In addition, some endocrine-like cells of the ileum were calbindin-D28K-positive. Half of these endocrine cells also contained neurotensin but none of the other neuropeptides investigated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calbindin 1
  • Calbindins
  • Enkephalin, Leucine / analysis
  • Enkephalin, Leucine / immunology
  • Immunohistochemistry
  • Intestines / cytology*
  • Intestines / innervation
  • Male
  • Neurosecretory Systems / analysis*
  • Neurosecretory Systems / cytology
  • Neurosecretory Systems / immunology
  • Neurotensin / analysis
  • Neurotensin / immunology
  • Rats
  • Rats, Inbred Strains
  • S100 Calcium Binding Protein G / analysis*
  • S100 Calcium Binding Protein G / immunology
  • Somatostatin / analysis
  • Somatostatin / immunology
  • Substance P / analysis
  • Substance P / immunology
  • Vasoactive Intestinal Peptide / analysis
  • Vasoactive Intestinal Peptide / immunology

Substances

  • Calb1 protein, rat
  • Calbindin 1
  • Calbindins
  • S100 Calcium Binding Protein G
  • Substance P
  • Vasoactive Intestinal Peptide
  • Neurotensin
  • Somatostatin
  • Enkephalin, Leucine