Ion-conducting channels in a gram-positive bacterium

FEBS Lett. 1988 Nov 21;240(1-2):105-9. doi: 10.1016/0014-5793(88)80348-x.

Abstract

The patch-clamp technique was used to obtain information on the existence and properties of ion channels in giant protoplasts obtained from the Gram-positive bacterium Streptococcus faecalis. The membrane proved to contain a pore with numerous conductance states, ranging from 10 pS to several nanosiemens. Application of a slight pressure differential across the membrane resulted in the activation of the channel. The pressure sensitivity points to a relationship between this channel and one recently discovered in E. coli spheroplasts [(1987) Proc. Natl. Acad. Sci. USA 84, 2297-2301] suggesting that pores of this type might be widespread among prokaryotes.

MeSH terms

  • Electric Conductivity
  • Electrophysiology
  • Enterococcus faecalis / physiology*
  • Hydrostatic Pressure
  • Ion Channels / physiology*

Substances

  • Ion Channels