Assignments of RNase A by ADAPT-NMR and enhancer

Biomol NMR Assign. 2015 Apr;9(1):81-8. doi: 10.1007/s12104-014-9549-z. Epub 2014 Mar 12.


We report here backbone (1)H and (15)N assignments for ribonuclease A obtained by using ADAPT-NMR, a fully-automated approach for combined data collection, spectral analysis and resonance assignment. ADAPT-NMR was able to assign 98% of the resonances with 93% agreement with traditional data collection and assignment. Further refinement of the automated results with ADAPT-NMR enhancer led to complete (100%) assignments with 96% agreement with assignments by the traditional approach.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Nuclear Magnetic Resonance, Biomolecular*
  • Phosphates / pharmacology
  • Ribonuclease, Pancreatic / chemistry*


  • Phosphates
  • Ribonuclease, Pancreatic