Plaque reduction neutralization antibody test does not accurately predict protection against dengue infection in Ratchaburi cohort, Thailand

Virol J. 2014 Mar 12;11:48. doi: 10.1186/1743-422X-11-48.

Abstract

Background: The plaque reduction neutralization test (PRNT) is currently the best and most widely accepted approach to measuring virus-neutralizing and protective antibodies to dengue virus, and in assessing the immunogenicity of a dengue vaccine. However, the correlation between presence of dengue-neutralizing antibody and protection from infection is not absolute.

Findings: In a cohort study in Ratchaburi Province, Thailand, 48 subjects with serologically confirmed symptomatic dengue infection were tested for pre-existing dengue neutralizing antibody using PRNT. Nine subjects had quite high pre-existing PRNT50 titers (titer >90) to subsequent infecting dengue serotypes, but still had symptomatic infections.

Conclusion: This report provides evidence that PRNT may not be a good test for predicting protection against subsequent dengue infection.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Antibodies, Neutralizing / blood*
  • Antibodies, Viral / blood*
  • Child
  • Cohort Studies
  • Dengue / immunology*
  • Dengue Virus / immunology*
  • Female
  • Humans
  • Male
  • Neutralization Tests / methods*
  • Thailand
  • Viral Plaque Assay / methods*

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral