The C-terminal repeat domains of nsP3 from the Old World alphaviruses bind directly to G3BP

J Virol. 2014 May;88(10):5888-93. doi: 10.1128/JVI.00439-14. Epub 2014 Mar 12.


The Old World alphaviruses block stress granule assembly by sequestration of RasGAP SH3-domain binding protein (G3BP). Here, we show that the proline-rich sequences in the hypervariable domain of nonstructural protein 3 (nsP3) of both Semliki Forest virus and Chikungunya virus were dispensable for binding to G3BP. nsP3 variants with or without this domain colocalized with G3BP. Furthermore, we show that the C-terminal repeat motifs of nsP3 were sufficient for G3BP binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chikungunya virus / physiology*
  • Host-Pathogen Interactions*
  • Semliki forest virus / physiology*
  • Viral Nonstructural Proteins / metabolism*


  • Viral Nonstructural Proteins