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. 2014 Apr 14;53(16):4107-12.
doi: 10.1002/anie.201308903. Epub 2014 Mar 12.

Direct and Single-Molecule Visualization of the Solution-State Structures of G-hairpin and G-triplex Intermediates

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Direct and Single-Molecule Visualization of the Solution-State Structures of G-hairpin and G-triplex Intermediates

Arivazhagan Rajendran et al. Angew Chem Int Ed Engl. .

Abstract

We present the direct and single-molecule visualization of the in-pathway intermediates of the G-quadruplex folding that have been inaccessible by any experimental method employed to date. Using DNA origami as a novel tool for the structural control and high-speed atomic force microscopy (HS-AFM) for direct visualization, we captured images of the unprecedented solution-state structures of a tetramolecular antiparallel and (3+1)-type G-quadruplex intermediates, such as G-hairpin and G-triplex, with nanometer precision. No such structural information was reported previously with any direct or indirect technique, solution or solid-state, single-molecule or bulk studies, and at any resolution. Based on our results, we proposed a folding mechanism of these G-quadruplexes.

Keywords: DNA origami; G-hairpin; G-quadruplex; G-triplex; atomic force microscopy.

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