Evaluation of the Architect Epstein-Barr Virus (EBV) viral capsid antigen (VCA) IgG, VCA IgM, and EBV nuclear antigen 1 IgG chemiluminescent immunoassays for detection of EBV antibodies and categorization of EBV infection status using immunofluorescence assays as the reference method

Clin Vaccine Immunol. 2014 May;21(5):684-8. doi: 10.1128/CVI.00104-14. Epub 2014 Mar 12.


Commercial immunoassays for detecting IgG and IgM antibodies against Epstein-Barr virus (EBV), viral capsid antigens (VCA), and IgGs toward EBV nuclear antigen-1 (EBNA-1) are routinely used in combination to categorize EBV infection status. In this study, we evaluated the performances of the Architect EBV VCA IgG, VCA IgM, and EBNA-1 IgG chemiluminescent microparticle assays (CMIAs) in EBV serological analyses using indirect immunofluorescence assays and anticomplement immunofluorescence assays as the reference methods for VCA IgG, VCA IgM, and EBNA-1 IgG antibody detection, respectively. A total of 365 serum samples representing different EBV serological profiles were included in this study. The κ values (concordances between the results) obtained in the Architect CMIA and those in the reference assays were 0.905 (P < 0.0001) for VCA IgM, 0.889 (P < 0.0001) for VCA IgG, and 0.961 (P < 0.0001) for EBNA-1 IgG. The sensitivities and specificities were, respectively, 91.08% and 99.48% for VCA IgM, 99.23% and 86.27% for VCA IgG, and 96.77% and 99.16% for EBNA-1 IgG. The sensitivities and specificities of the Architect CMIA panel were, respectively, 99.15% and 98.6% for diagnosing a primary infection, 97.62% and 93.39% for diagnosing a past EBV infection, and 92.42% and 97.82% for diagnosing the absence of an EBV infection. In summary, we demonstrated that the Architect EBV antibody panel performs very well for EBV antibody detection and correctly categorizes clinically relevant EBV infection states.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Antibodies, Viral / blood*
  • Antigens, Viral
  • Capsid Proteins*
  • Child
  • Child, Preschool
  • Diagnostic Tests, Routine / methods
  • Epstein-Barr Virus Infections / diagnosis*
  • Epstein-Barr Virus Nuclear Antigens*
  • Female
  • Fluorescent Antibody Technique / methods
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Immunoassay / methods
  • Immunoglobulin G / blood*
  • Immunoglobulin M / blood*
  • Infant
  • Luminescent Measurements / methods
  • Male
  • Sensitivity and Specificity


  • Antibodies, Viral
  • Antigens, Viral
  • Capsid Proteins
  • Epstein-Barr Virus Nuclear Antigens
  • Immunoglobulin G
  • Immunoglobulin M
  • EBV-encoded nuclear antigen 1