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. Spring 2013;8(2):76-80.
Epub 2013 May 4.

Preventive Effects of Pomegranate Seed Extract on Bleomycin-Induced Pulmonary Fibrosis in Rat

Free PMC article

Preventive Effects of Pomegranate Seed Extract on Bleomycin-Induced Pulmonary Fibrosis in Rat

Ali Asghar Hemmati et al. Jundishapur J Nat Pharm Prod. .
Free PMC article


Background: Oxidative stress plays an important role in the pathogenesis of bleomycin-induced lung fibrosis and many antioxidant agents have been used for the treatment of this disease in animals.

Objectives: To evaluate the antioxidant effects of pomegranate seed extract (PSE) on bleomycin treated rats.

Materials and methods: Male Spraque - Dawley rats were divided into 5 groups: rats in groups I (bleomycin) and II (control) were given a single dose of bleomycin (7.5 IU/kg, intratracheally) in the bleomycin group and same amount of saline in the control, respectively. Treatment groups (III-V) were given PSE (100,200,400 mg/kg) orally a week before the bleomycin injection and this was continued for 3 weeks. At day 28, animals were sacrificed and lungs were removed for histological investigation.

Results: Histological analysis showed that PSE could prevent pathological changes that were seen in the bleomycin group.

Conclusions: Results of the present study showed that hydroalcoholic extracts of pomegranate seeds had a significant protective effect against bleomycin-induced lung fibrosis by its antioxidant properties. The highest protective effect was observed for the 400 mg/kg dose.

Keywords: Antioxidants; Bleomycin; Pulmonary Fibrosis.


Figure 1.
Figure 1.. The Influence of PSE on Histopathological Changes in Rats with BLM-induced Lung Injury. A. Normal Lung Histology in the Saline Group; B. Inflammatory Cell Infiltration and Fibrosis in the BLM Group; C. Weaker Fibrosis in the PSE Group (100 mg/kg); D. Reduced Lung Fibrosis in the PSE Group (200 mg/kg); E. Marked Prevention of Fibrosis in the PSE Group (400 mg/kg).

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