Evaluation of the antioxidant and anti-osteoporosis activities of chemical constituents of the fruits of Prunus mume

Food Chem. 2014 Aug 1:156:408-15. doi: 10.1016/j.foodchem.2014.01.078. Epub 2014 Feb 6.

Abstract

The present study investigated the antioxidant and anti-osteoporosis activities of phytochemicals in the fruits of Prunus mume. From the methanol extract, three new acylated sucroses, mumeoses P-R (1-3), were isolated together with 20 known compounds (4-23). Compounds 1-3 showed potent peroxyl radical-scavenging activities and 12-19 showed both potent peroxyl radical-scavenging and reducing activities. The anti-osteoporosis activity was evaluated using murine pre-osteoblastic MC3T3-E1 cells and pre-osteoclastic RAW 264.7 cells. Compounds 2 and 3 (cis-trans isomers), 5, 7, 8, and 10 significantly stimulated the differentiation of pre-osteoblastic MC3T3-E1 cells to increase collagen synthesis or mineralization functions of osteoblasts, while compounds 5, 6, 9, 10, 12, 14-16, 18, 20, and 22 significantly suppressed tartrate-resistant acid phosphatase activity in receptor activator of nuclear factor-κB ligand-induced osteoclastic RAW 264.7 cells. These results indicated that the fruits of P. mume are an excellent source of antioxidant and anti-osteoporosis phytochemicals.

Keywords: Acylated sucrose; Anti-osteoporosis activity; Antioxidant activity; Prunus mume; Rosaceae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Acid Phosphatase / chemistry*
  • Acylation
  • Animals
  • Antioxidants / chemistry*
  • Cell Differentiation / drug effects
  • Collagen / chemistry
  • Fruit / chemistry*
  • Isoenzymes / chemistry*
  • Mice
  • Osteoblasts / drug effects
  • Osteoporosis / physiopathology
  • Osteoporosis / prevention & control*
  • Plant Extracts / pharmacology*
  • Prunus / chemistry*
  • Tartrate-Resistant Acid Phosphatase

Substances

  • Antioxidants
  • Isoenzymes
  • Plant Extracts
  • Collagen
  • Acid Phosphatase
  • Tartrate-Resistant Acid Phosphatase