Overexpression of B7-H3 in CD133+ colorectal cancer cells is associated with cancer progression and survival in human patients

J Surg Res. 2014 May 15;188(2):396-403. doi: 10.1016/j.jss.2014.01.014. Epub 2014 Jan 24.

Abstract

Cancer stem-like cells are enriched in CD133-positive (CD133(+)) colorectal cancer (CRC) cells. To date, the biological significance of CD133 expression in cancer stem-like cells is still unknown. B7-H3, a costimulatory molecule, plays a pivotal role in tumor immune escape by inhibiting the functions of T cells. To identify a new marker to predict the tumor grade of CRC, we analyzed the expression of B7-H3 and CD133 in colorectal tumor samples, and their clinical significance was determined. By using a series of techniques including pathologic tissue microarray technology, immunohistochemistry, and immunofluorescent staining, we found B7-H3 was expressed in 56.73% of the CRC cases (59/104) sampled; CD133 was detected in 26.92% of the CRC cases (28/104) sampled. Further analysis indicated that 22 of these CD133(+) samples expressed B7-H3. We also found coexpression of CD133 and B7-H3 in tumor tissue samples (r = 0.321, P < 0.01). Moreover, in contrast to individual CD133 or B7-H3 expression, the coexpression of B7-H3 and CD133 was evidently associated with the depth of tumor invasion, lymphatic metastasis, distant metastasis, and Dukes' stage, suggesting it is a valuable biomarker for the progression of CRC. Indeed, the patients with coexpression of B7-H3 and CD133 had a poorer survival than the other patients (P < 0.05). In summary, our results reveal that B7-H3 was aberrantly expressed in CD133(+) CRC cells, and the expression level was closely associated with tumor progression.

Keywords: B7-H3; CD133; Clinicopathology; Colorectal cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AC133 Antigen
  • Adenocarcinoma / genetics*
  • Adenocarcinoma / mortality
  • Adenocarcinoma / pathology*
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, CD / biosynthesis
  • Antigens, CD / genetics*
  • B7 Antigens / biosynthesis
  • B7 Antigens / genetics*
  • B7 Antigens / metabolism
  • Colon / pathology
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / mortality
  • Colorectal Neoplasms / pathology*
  • Disease Progression
  • Female
  • Glycoproteins / biosynthesis
  • Glycoproteins / genetics*
  • Humans
  • Lymphatic Metastasis / genetics
  • Male
  • Middle Aged
  • Peptides / genetics*
  • Rectum / pathology

Substances

  • AC133 Antigen
  • Antigens, CD
  • B7 Antigens
  • CD276 protein, human
  • Glycoproteins
  • PROM1 protein, human
  • Peptides