Somatic Antigens of Shigella: Structure of the O-specific Polysaccharide Chain of the Shigella Dysenteriae Type 7 Lipopolysaccharide

Carbohydr Res. 1988 Aug 15;179:51-60. doi: 10.1016/0008-6215(88)84109-0.

Abstract

4-(N-Acetylglycyl)amino-4,6-dideoxy-D-glucose has been identified as a component of the Shigella dysenteriae type 7 O-specific polysaccharide, in addition to the previously reported 2-acetamido-2-deoxy-D-glucose and 2-acetamido-2-deoxy-D-galacturonic acid. On the basis of selective cleavage with anhydrous hydrogen fluoride and analysis by 1H- and 13C-n.m.r. spectroscopy and f.a.b.-mass spectrometry, it was concluded that the tetrasaccharide repeating-unit of the polysaccharide has the following structure: (structure; see text) where D-GalNAcAN is 2-acetamido-2-deoxy-D-galacturonamide and D-Qui4N is 4-amino-4,6-dideoxy-D-glucose.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, Bacterial* / analysis
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Carbohydrates / analysis
  • Chemical Phenomena
  • Chemistry
  • Hydrolysis
  • Lipopolysaccharides* / analysis
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Molecular Sequence Data
  • Molecular Structure
  • O Antigens
  • Pseudomonas aeruginosa / immunology
  • Shigella dysenteriae / immunology*

Substances

  • Antigens, Bacterial
  • Carbohydrates
  • Lipopolysaccharides
  • O Antigens