Distinct dendritic cell subsets dictate the fate decision between effector and memory CD8(+) T cell differentiation by a CD24-dependent mechanism

Immunity. 2014 Mar 20;40(3):400-13. doi: 10.1016/j.immuni.2014.02.004. Epub 2014 Mar 13.


The contribution of different DC subsets to effector and memory CD8(+) T cell generation during infection and the mechanism by which DCs controls these fate decisions is unclear. Here we demonstrated that the CD103(+) and CD11b(hi) migratory respiratory DC (RDC) subsets after influenza virus infection activated naive virus-specific CD8(+) T cells differentially. CD103(+) RDCs supported the generation of CD8(+) T effector (Teff) cells, which migrate from lymph nodes to the infected lungs. In contrast, migrant CD11b(hi) RDCs activated CD8(+) T cells characteristic of central memory CD8(+) T (CD8(+) Tcm) cells including retention within the draining lymph nodes. CD103(+) RDCs expressed CD24 at an elevated level, contributing to the propensity of this DC subpopulation to support CD8(+) Teff cell differentiation. Mechanistically, CD24 was shown to regulate CD8(+) T cell activation through HMGB1-mediated engagement of T cell RAGE. Thus, there is distribution of labor among DC subsets in regulating CD8(+) T cell differentiation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antigens, CD / metabolism
  • CD11b Antigen / metabolism
  • CD24 Antigen / metabolism*
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism*
  • CD8-Positive T-Lymphocytes / virology
  • Cell Differentiation / immunology*
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Female
  • Immunologic Memory*
  • Immunophenotyping
  • Integrin alpha Chains / metabolism
  • Lung / immunology
  • Lung / metabolism
  • Lung / virology
  • Lymph Nodes / immunology
  • Lymph Nodes / metabolism
  • Lymph Nodes / virology
  • Lymphocyte Activation / immunology
  • Mice
  • Mice, Knockout
  • Phenotype
  • Protein Binding
  • Receptor for Advanced Glycation End Products
  • Receptors, Immunologic / metabolism
  • Virus Release / immunology


  • Antigens, CD
  • CD11b Antigen
  • CD24 Antigen
  • Integrin alpha Chains
  • Receptor for Advanced Glycation End Products
  • Receptors, Immunologic
  • alpha E integrins