Sour cherry (Prunus cerasus) seed extract increases heme oxygenase-1 expression and decreases proinflammatory signaling in peripheral blood human leukocytes from rheumatoid arthritis patients

Fadia Mahmoud; David Haines; Rana Al-Awadhi; Ali A Dashti; Adel Al-Awadhi; Basel Ibrahim; Bashayer Al-Zayer; Bela Juhasz; Arpad Tosaki

doi:10.1016/j.intimp.2014.02.031

Sour cherry (Prunus cerasus) seed extract increases heme oxygenase-1 expression and decreases proinflammatory signaling in peripheral blood human leukocytes from rheumatoid arthritis patients

Int Immunopharmacol. 2014 May;20(1):188-96. doi: 10.1016/j.intimp.2014.02.031. Epub 2014 Mar 12.

Authors

Fadia Mahmoud¹, David Haines², Rana Al-Awadhi³, Ali A Dashti³, Adel Al-Awadhi⁴, Basel Ibrahim³, Bashayer Al-Zayer³, Bela Juhasz², Arpad Tosaki²

Affiliations

¹ Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait. Electronic address: fadia@hsc.edu.kw.
² Debrecen University, Faculty of Pharmacy, Hungary.
³ Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait.
⁴ Department of Medicine, Faculty of Medicine, Kuwait University, Kuwait.

PMID: 24631368
DOI: 10.1016/j.intimp.2014.02.031

Abstract

Sour cherry seed extract (SCE) was evaluated for its capacity to inhibit lipopolysaccharide-treated human peripheral blood T cells expressing tumor necrosis factor-alpha, and the chemokine interleukin-8. Both proteins are diagnostic biomarkers for inflammatory pathologies. Peripheral blood leukocytes from 11 rheumatoid arthritis (RA) patients and 8 healthy control subjects were co-cultured for 24h in lipopolysaccharide and the extract, then evaluated by flow cytometry for T cell activation and by enzyme-linked immunoassay for lymphocyte-associated heme oxygenase-1 (HO-1) expression. There was a dose-dependent decrease in expression of the immunophenotypes: CD3+TNF-α+, and CD3+IL8+ in cultures from RA patients to a greater extent than in cells from healthy participants. These results suggest that the extract may have a modulatory roll in RA and other inflammatory disorders via the induction of HO-1, thus abating oxidative stress and strengthening regulation of pro-inflammatory signaling pathways.

Keywords: HO-1; IL-8; Peripheral blood; Rheumatoid arthritis; Sour cherry kernel; TNF-α.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Arthritis, Rheumatoid / immunology*
CD3 Complex / immunology
Cells, Cultured
Female
Heme Oxygenase-1 / immunology*
Humans
Interleukin-8 / immunology
Leukocytes, Mononuclear / drug effects*
Leukocytes, Mononuclear / immunology
Lipopolysaccharides
Middle Aged
Plant Extracts / pharmacology*
Prunus*
Seeds
T-Lymphocytes / drug effects*
T-Lymphocytes / immunology
Tumor Necrosis Factor-alpha / immunology

Substances

CD3 Complex
CXCL8 protein, human
Interleukin-8
Lipopolysaccharides
Plant Extracts
Tumor Necrosis Factor-alpha
HMOX1 protein, human
Heme Oxygenase-1