B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties

EMBO J. 1988 Nov;7(11):3457-64.

Abstract

A gene, called m-mb-1, was isolated from a murine pre-B-minus T lymphocyte subtracted library. It was found expressed as mRNA at low to medium abundance in early progenitors of the B lineage, in pre-B and mature B lineage cell lines, in normal resting B lymphocytes and in polyclonally activated B cell blasts. The gene was not expressed in plasmacytomas, in cell lines of the monocyte/macrophage, the T lymphocyte or the fibroblast lineages, nor in thymus, liver, heart, kidney, lung or brain. The nucleotide sequence of the m-mb-1 gene encodes a putative membrane glycoprotein with 220 amino acids, which includes a leader sequence, a putative extracellular domain with two potential N-glycosylation sites, a transmembrane portion and a putative intracellular domain. The partial sequence of a human homologue, h-mb-1, shows nearly 90% homology in nucleotide as well as amino acid sequences to the murine form of a stretch of the putative intracytoplasmic domain. Antibodies raised against a fusion protein of m-mb-1 with protein A, affinity purified for their m-mb-1 specificity, stained pre-B and mature B cell lines on their surface, but did not stain T cell lines and fibroblasts. Antibodies raised against a stretch of 20 amino acids of the putative intracellular domain with 90% homology between the mouse and human protein did not stain the surface of any cell lines tested.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation, T-Lymphocyte / biosynthesis
  • Antigens, Differentiation, T-Lymphocyte / genetics*
  • B-Lymphocytes / immunology*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • CD3 Complex
  • Cell Line
  • Cells, Cultured
  • DNA / genetics
  • Gene Expression Regulation*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Plasmacytoma
  • RNA / genetics
  • Receptors, Antigen, T-Cell / biosynthesis
  • Receptors, Antigen, T-Cell / genetics*
  • Restriction Mapping
  • Transcription, Genetic
  • Tumor Cells, Cultured

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • CD3 Complex
  • Receptors, Antigen, T-Cell
  • RNA
  • DNA