Development of hydrogel biochip for in vitro allergy diagnostics

J Immunol Methods. 2014 Apr;406:51-7. doi: 10.1016/j.jim.2014.03.003. Epub 2014 Mar 12.

Abstract

A hydrogel biochip was developed for the simultaneous quantitative determination of sIgE for 21 allergens and total IgE in human serum. The biochips are manufactured by photoinduced copolymerization of different molecules (allergens and antibodies) with gel-forming monomers resulting in the formation of three-dimensional hydrogel elements (1nl gel drops). After incubation of the biochip with the serum, the results are visualized using fluorescently labeled anti-IgE antibodies. Using biochips, serum samples from allergic patients and healthy donors were analyzed and good correlation with the results obtained using commercial EIA test systems of generally recognized quality (Dr. Fooke Laboratorien GmbH, Germany) was observed.

Keywords: Allergy diagnostics; Biochip; IgE in vitro test; Protein microarray.

MeSH terms

  • Allergens / immunology
  • Antibodies, Anti-Idiotypic* / immunology
  • Antigens / immunology
  • Fluorescent Antibody Technique
  • Humans
  • Hydrogels / chemical synthesis*
  • Hypersensitivity / blood
  • Hypersensitivity / diagnosis*
  • Hypersensitivity / immunology
  • Immunoglobulin E / blood*
  • Immunoglobulin E / immunology
  • Protein Array Analysis / methods*
  • Skin Tests

Substances

  • Allergens
  • Antibodies, Anti-Idiotypic
  • Antigens
  • Hydrogels
  • anti-IgE antibodies
  • Immunoglobulin E