Coordinate induction of metallothioneins I and II in rodent cells by UV irradiation

Mol Cell Biol. 1988 Nov;8(11):4716-20. doi: 10.1128/mcb.8.11.4716-4720.1988.


Sequence analysis of Chinese hamster V79 lung fibroblast cDNA clones, which code for UV radiation-inducible transcripts, revealed that many of the clones corresponded to metallothioneins (MTs) I and II. A third cDNA clone, DDIU4, was found also to code for a similar-size UV-inducible transcript which was unrelated to MT by both sequence analysis and kinetics of induction. MTI and MTII RNAs rapidly increased in V79 cells within 1 h after UV irradiation, and maximum induction was seen by 4 h. This rapid induction of MT RNA by UV irradiation was not observed in human fibroblasts. MTI and MTII were coordinately induced in both time course and dose-response experiments, although the induction of MTII, up to 30-fold, was three to four times greater than that of MTI. The induction of MT did not appear to be a general stress response, since no increase occurred after exposure to X rays or H2O2.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • DNA / genetics
  • DNA Damage
  • Kinetics
  • Metallothionein / biosynthesis*
  • Metallothionein / genetics
  • Molecular Sequence Data
  • RNA / biosynthesis
  • RNA / genetics
  • RNA / radiation effects
  • Ultraviolet Rays*


  • RNA
  • DNA
  • Metallothionein

Associated data

  • GENBANK/M22350