Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells

Proc Natl Acad Sci U S A. 1989 Jan;86(2):645-9. doi: 10.1073/pnas.86.2.645.

Abstract

Monoclonal antibodies specific for the murine CD2 antigen were identified by an efficient screening method utilizing murine CD2 cDNA transfectants. An unexpected expression of CD2 on murine B cells was revealed by immunofluorescence and immunoprecipitation studies with these monoclonal antibodies and by RNA blot analysis for the murine CD2 transcript.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Antigens, Differentiation, T-Lymphocyte / analysis*
  • Antigens, Differentiation, T-Lymphocyte / genetics
  • Antigens, Surface / analysis
  • Antigens, Surface / genetics
  • B-Lymphocytes / immunology*
  • Blotting, Northern
  • CD2 Antigens
  • Cell Line
  • Cricetinae
  • DNA / genetics
  • Enzyme-Linked Immunosorbent Assay
  • Fibroblasts
  • Fluorescent Antibody Technique
  • Immune Sera / immunology
  • Precipitin Tests
  • RNA / analysis
  • Rats
  • Receptors, Immunologic / analysis*
  • Receptors, Immunologic / genetics
  • T-Lymphocytes / immunology*
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • CD2 Antigens
  • Immune Sera
  • Receptors, Immunologic
  • RNA
  • DNA