Taking it one step at a time in homologous recombination repair

Humberto Sanchez; Marcel Reuter; Masatoshi Yokokawa; Kunio Takeyasu; Claire Wyman

doi:10.1016/j.dnarep.2014.02.012

Taking it one step at a time in homologous recombination repair

DNA Repair (Amst). 2014 Aug:20:110-118. doi: 10.1016/j.dnarep.2014.02.012. Epub 2014 Mar 11.

Authors

Humberto Sanchez¹, Marcel Reuter¹, Masatoshi Yokokawa², Kunio Takeyasu³, Claire Wyman⁴

Affiliations

¹ Department of Genetics, Cancer Genomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands.
² Graduate School of Pure and Applied Science, University of Tsukuba, Japan.
³ Kyoto University Graduate School of Biostudies, Japan.
⁴ Department of Genetics, Cancer Genomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands; Department of Radiation Oncology, Erasmus University Medical Center, Rotterdam, The Netherlands. Electronic address: c.wyman@erasmusmc.nl.

PMID: 24636751
DOI: 10.1016/j.dnarep.2014.02.012

Abstract

The individual steps in the process of homologous recombination are particularly amenable to analysis by single-molecule imaging and manipulation experiments. Over the past 20 years these have provided a wealth of new information on the DNA transactions that make up this vital process. Exciting progress in developing new tools and techniques to analyze more complex components, dynamic reaction steps and molecular coordination continues at a rapid pace. Here we highlight recent results and indicate some emerging techniques likely to produce the next stage of advanced insight into homologous recombination. In this and related fields the future is bright.

Keywords: High-speed AFM; Scanning force microscopy; TIRF-SFM.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Humans
Microscopy, Atomic Force / methods*
Optical Tweezers*
Recombinational DNA Repair*