Repeated TALEs: visualizing DNA sequence localization and chromosome dynamics in live cells

Nucleus. 2014 Jan-Feb;5(1):28-31. doi: 10.4161/nucl.28143. Epub 2014 Feb 10.

Abstract

Three recent papers, published just weeks apart, describe the use of fluorescent TALEs to tag specific DNA sequences in live cells and, in one case, also in fixed cells, the latter with potential clinical applications.

Keywords: DNA replication timing; cell cycle; centromeres; single nucleotide polymorphisms; telomeres; transcription activator-like effectors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Comment

MeSH terms

  • Animals
  • Cell Nucleus / chemistry*
  • Cell Nucleus / metabolism*
  • Chromatin / metabolism*
  • Chromosomes, Human / genetics*
  • DNA, Satellite / analysis*
  • DNA-Binding Proteins*
  • Fluorescent Dyes / metabolism*
  • Humans
  • Microscopy, Fluorescence / methods*
  • Repetitive Sequences, Nucleic Acid / genetics*
  • Staining and Labeling / methods*
  • Transcriptional Activation / genetics*

Substances

  • Chromatin
  • DNA, Satellite
  • DNA-Binding Proteins
  • Fluorescent Dyes