Abstract
Three recent papers, published just weeks apart, describe the use of fluorescent TALEs to tag specific DNA sequences in live cells and, in one case, also in fixed cells, the latter with potential clinical applications.
Keywords:
DNA replication timing; cell cycle; centromeres; single nucleotide polymorphisms; telomeres; transcription activator-like effectors.
Publication types
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Research Support, Non-U.S. Gov't
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Comment
MeSH terms
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Animals
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Cell Nucleus / chemistry*
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Cell Nucleus / metabolism*
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Chromatin / metabolism*
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Chromosomes, Human / genetics*
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DNA, Satellite / analysis*
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DNA-Binding Proteins*
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Fluorescent Dyes / metabolism*
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Humans
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Microscopy, Fluorescence / methods*
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Repetitive Sequences, Nucleic Acid / genetics*
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Staining and Labeling / methods*
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Transcriptional Activation / genetics*
Substances
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Chromatin
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DNA, Satellite
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DNA-Binding Proteins
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Fluorescent Dyes