Effect of eccentric versus concentric exercise training on mitochondrial function

Muscle Nerve. 2014 Nov;50(5):803-11. doi: 10.1002/mus.24215. Epub 2014 Aug 30.


Introduction: The effect of eccentric (ECC) versus concentric (CON) training on metabolic properties in skeletal muscle is understood poorly. We determined the responses in oxidative capacity and mitochondrial H2 O2 production after eccentric (ECC) versus concentric (CON) training performed at similar mechanical power.

Methods: Forty-eight rats performed 5- or 20-day eccentric (ECC) or concentric (CON) training programs. Mitochondrial respiration, H2 O2 production, citrate synthase activity (CS), and skeletal muscle damage were assessed in gastrocnemius (GAS), soleus (SOL) and vastus intermedius (VI) muscles.

Results: Maximal mitochondrial respiration improved only after 20 days of concentric (CON) training in GAS and SOL. H2 O2 production increased specifically after 20 days of eccentric ECC training in VI. Skeletal muscle damage occurred transiently in VI after 5 days of ECC training.

Conclusions: Twenty days of ECC versus CON training performed at similar mechanical power output do not increase skeletal muscle oxidative capacities, but it elevates mitochondrial H2 O2 production in VI, presumably linked to transient muscle damage.

Keywords: eccentric exercise; mitochondria; oxidative capacities; reactive oxygen species; skeletal muscle.

MeSH terms

  • Adenosine Diphosphate / metabolism
  • Animals
  • Body Mass Index
  • Citrate (si)-Synthase / metabolism
  • Creatine Kinase / metabolism
  • Hydrogen Peroxide / metabolism
  • Lactic Acid / blood
  • Male
  • Maximal Voluntary Ventilation
  • Mitochondria, Muscle / physiology*
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / ultrastructure*
  • Oxidative Stress / physiology*
  • Physical Conditioning, Animal / physiology*
  • Rats
  • Rats, Wistar
  • Succinic Acid
  • Time Factors


  • Lactic Acid
  • Adenosine Diphosphate
  • Succinic Acid
  • Hydrogen Peroxide
  • Citrate (si)-Synthase
  • Creatine Kinase