Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258 Klebsiella pneumoniae

Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4988-93. doi: 10.1073/pnas.1321364111. Epub 2014 Mar 17.


Infections caused by drug-resistant bacteria are a major problem worldwide. Carbapenem-resistant Klebsiella pneumoniae, most notably isolates classified as multilocus sequence type (ST) 258, have emerged as an important cause of hospital deaths. ST258 isolates are predominantly multidrug resistant, and therefore infections caused by them are difficult to treat. It is not known why the ST258 lineage is the most prevalent cause of multidrug-resistant K. pneumoniae infections in the United States and other countries. Here we tested the hypothesis that carbapenem-resistant ST258 K. pneumoniae is a single genetic clone that has disseminated worldwide. We sequenced to closure the genomes of two ST258 clinical isolates and used these genomes as references for comparative genome sequencing of 83 additional clinical isolates recovered from patients at diverse geographic locations worldwide. Phylogenetic analysis of the SNPs in the core genome of these isolates revealed that ST258 K. pneumoniae organisms are two distinct genetic clades. This unexpected finding disproves the single-clone hypothesis. Notably, genetic differentiation between the two clades results from an ∼ 215-kb region of divergence that includes genes involved in capsule polysaccharide biosynthesis. The region of divergence appears to be a hotspot for DNA recombination events, and we suggest that this region has contributed to the success of ST258 K. pneumoniae. Our findings will accelerate research on novel diagnostic, therapeutic, and vaccine strategies designed to prevent and/or treat infections caused by multidrug resistant K. pneumoniae.

Keywords: Enterobacteriaceae; antibiotic resistance; carbapenemase; plasmid.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Base Pairing / genetics
  • Base Sequence
  • Carbapenems / pharmacology*
  • Drug Resistance, Multiple, Bacterial / drug effects*
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Evolution, Molecular*
  • Genome, Bacterial / genetics
  • Geography
  • Humans
  • Klebsiella pneumoniae / classification*
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Molecular Sequence Data
  • Multilocus Sequence Typing*
  • Phylogeny
  • Plasmids / genetics
  • Polymorphism, Single Nucleotide / genetics
  • Sequence Analysis, DNA


  • Carbapenems

Associated data

  • GENBANK/CP006918
  • GENBANK/CP006919
  • GENBANK/CP006921
  • GENBANK/CP006922
  • GENBANK/CP006923
  • GENBANK/CP006924
  • GENBANK/CP006925
  • GENBANK/CP006926
  • GENBANK/CP006927
  • GENBANK/CP006928
  • SRA/SRP036874