Case control study of the factor V Leiden and factor II G20210A mutation frequency in women with recurrent pregnancy loss

Majid Teremmahi Ardestani; Hossein Hadi Nodushan; Abbas Aflatoonian; Nasrin Ghasemi; Mohammad Hasan Sheikhha

Case control study of the factor V Leiden and factor II G20210A mutation frequency in women with recurrent pregnancy loss

Iran J Reprod Med. 2013 Jan;11(1):61-4.

Authors

Majid Teremmahi Ardestani¹, Hossein Hadi Nodushan¹, Abbas Aflatoonian², Nasrin Ghasemi³, Mohammad Hasan Sheikhha³

Affiliations

¹ Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
² Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
³ Department of Medical Genetics, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

PMID: 24639694
PMCID: PMC3941375

Abstract

Background: Recurrent pregnancy loss (RPL) caused by various genetic and non-genetic factors. After chromosome abnormality, thrombophilia is one of the most important genetic factors that could cause RPL. Factor V Leiden and factor II G20210A mutation were the most common mutations cause thrombophilia in the world.

Objective: The purpose of this study was to determine the frequency of factor V Leiden and prothrombine gene mutations in women with RPL compared with women who had uneventful pregnancies.

Materials and methods: This case control study evaluates the frequency of factor V-Leiden and factor II G20210 genotypes in 80 women with two or more pregnancy losses, compared with 80 women without adverse pregnancy outcome. The mutations were assessed by PCR-RFLP.

Results: Frequency of the factor V Leiden among cases was 2.5%, which was higher than controls (1.25%), but the difference was not significant. No factor II G20210 mutation was found among cases and controls.

Conclusion: These data did not confirm that factor V Leiden and factor II G20210 mutation might play a role in recurrent pregnancy loss in Iranian women.

Keywords: Factor II G20210A Mutation; Factor V Leiden; Recurrent Abortion.