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. 2013 May;11(5):415-22.

Curcumin Inhibits Endometriosis Endometrial Cells by Reducing Estradiol Production

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Free PMC article

Curcumin Inhibits Endometriosis Endometrial Cells by Reducing Estradiol Production

Ying Zhang et al. Iran J Reprod Med. .
Free PMC article

Abstract

Background: Endometriosis is a complex estrogen-dependent disease that is defined as the presence of endometrial gland and stroma outside the uterine cavity. Although the exact mechanism for the development of endometriosis remains unclear, there is a large body of research data and circumstantial evidence that suggests a crucial role of estrogen in the establishment and maintenance of this disease.

Objective: This study is an attempt to assess the effect of curcumin on inhibiting endometriosis endometrial cells and to investigate whether such an effect is mediated by reducing estradiol production.

Materials and methods: Endometriotic stromal cells, normal endometrial stromal cells, endometriotic epithelial cells and normal endometrial epithelial cells were isolated and cultured. E2 value of cells and the effect of curcumin on cell proliferation were evaluated. Finally, effect of curcumin on E2 assay was detected.

Results: Electrochemiluminescence immunoassay results showed that E2 value of endometriotic epithelial cells was higher than the endometriotic stromal cells (p=0.037), while the expression of E2 in normal endometrial stromal and epithelial cells was extremely low. WST-8 result showed, compared with endometrial stromal cells, ectopic endometriotic stromal cells had a higher growth rate. After intervene with curcumin (10μmol/L, 30μmol/L and 50μmol/L) for 0-96h, the number of endometriotic stromal cells was reduced and cells growth slowed, compared with 0μmol/L group. Compared with 0μmol/L group, E2 level was lower after treatment with curcumin, especially in 30μmol/L and 50μmol/L group.

Conclusion: In summary, in this study we found that E2 is important in ectopic endometrium, and epithelial cell is in dominant position with E2 secretion. Curcumin was able to suppress the proliferation of endometrial cells by reducing the E2 value.

Keywords: Curcumin; Endometriosis; Epithelial cell; Estradiol; Stromal cell.

Figures

Figure 1
Figure 1
Chemical structural formula of curcumin (C21H20O6
Figure 2
Figure 2
Epithelial cells were polygonal, with thin and transparent cytoplasm and center located round nuclear. After 3-4 days culture, cells developed the appearance of decidual cell-like transformation with larger size. The cytoplasms were large and bright and the size of nucleus significantly increased. Cells were arranged in spiral-like or dough pattern with gradually outward derivative, and showing filamentous connection (A). Stromal cells had fibroblast morphology, spindle-shaped, abundant cytoplasm, oval-shaped nuclear. They were easy to passage. After long-term culture, these cells were arranged in parallel bundles, with dense piles of regional aggregation (B).
Figure 3
Figure 3
Electrochemiluminescence immunoassay results showed that E2 value of endometriotic epithelial cells was 12.90±0.6pg/ml, higher than that of the endometriotic stromal cells which is 9.34±1.37 pg/ml. There was a significant difference between them (p=0.037); E2 values of normal endometrial stromal cells and normal endometrial epithelial cells are extremely low, almost close to the minimum value of detection
Figure 4
Figure 4
Compared with endometrial stromal cells, ectopic endometrial stromal cells with the corresponding time points had a higher cell number. After intervene with curcumin (10μmol/L, 30μmol/L and 50μmol/L) for 96h, the number of endometriotic stromal cells was reduced, compared with 0μmol/L group
Figure 5
Figure 5
E2 level of endometriotic stromal cells was increased with time. Compared with previous time point, E2 level was increased by 33.18% (24hr), 57.28% (48hr), 38.80% (72hr) and 12.45% (96hr). Compared with 0μmol/L group, E2 level was lower after intervene with curcumin, especially in 30μmol/L and 50μmol/L groups (Cur 10μmol/L vs. Cur 0μmol/L p=0.094, Cur 30μmol/L vs. Cur 0μmol/L p=0.045, Cur 50μmol/L vs. Cur 0μmol/L p=0.026

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