[The anti-viral activity of the complex glycyrrhizic acid-alpha-glutamyl-tryptophan against experimental lethal influenza infection in white mice caused by oseltamivir-resistant strain of the virus]

Vopr Virusol. 2013 Sep-Oct;58(5):19-26.
[Article in Russian]

Abstract

Influenza virus is a leading causing factor of infectious respiratory human pathology. The ability to implement the antigenic drift and development of drug resistance makes it important to develop novel anti-influenza drugs of wide spectrum of activity. In this work, we present the results of the study of the activity of a combination of glycyrrhizic acid with dipeptide alpha-glutamyl-tryptophan against oseltamivir-reistant strain of the virus Al Vladivostok/2/09 (H1 N1) on the model of lethal influenza infection in white mice. Application of Orvilax was shown to decrease the specific mortality of animals (index of protection 39-67% depending on the dose of the virus and drugs combination), to increase the mean day of death to 3.7-5.0 days and decrease the infectious titer of the virus in lung tissue to 1.3 Ig EID50/20 mg. The corresponding figures for the reference compound Tamiflu were 8-11%, 0.5-1.5 days, and 0.6 Ig EID50/20 mg. The use of Orvilax also led to reliable increase of the titers of interferon in the blood from 30.4 to 56.5 ME/mL. The results obtained allow the drug to be considered as a promising anti-influenza remedy that is active against the drug-resistant virus strains.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Animals, Outbred Strains
  • Antiviral Agents / pharmacology*
  • Dipeptides / pharmacology*
  • Drug Combinations
  • Drug Resistance, Viral / drug effects
  • Drug Therapy, Combination
  • Female
  • Glycyrrhizic Acid / pharmacology*
  • Influenza A Virus, H1N1 Subtype / drug effects*
  • Influenza A Virus, H1N1 Subtype / immunology
  • Influenza A Virus, H1N1 Subtype / pathogenicity
  • Interferons / biosynthesis
  • Interferons / blood
  • Lung / drug effects*
  • Lung / pathology
  • Lung / virology
  • Mice
  • Orthomyxoviridae Infections / drug therapy*
  • Orthomyxoviridae Infections / immunology
  • Orthomyxoviridae Infections / mortality
  • Orthomyxoviridae Infections / virology
  • Oseltamivir / pharmacology
  • Survival Analysis
  • Viral Load / drug effects

Substances

  • Antiviral Agents
  • Dipeptides
  • Drug Combinations
  • Oseltamivir
  • alpha-glutamyltryptophan
  • Glycyrrhizic Acid
  • Interferons