Cloning and Expression of the Growth Hormone-Dependent Insulin-Like Growth Factor-Binding Protein

Mol Endocrinol. 1988 Dec;2(12):1176-85. doi: 10.1210/mend-2-12-1176.

Abstract

N-terminal as well as internal amino acid sequence data were obtained from the GH dependent, insulin-like growth factor (IGF) binding protein, BP-53, purified from human plasma. Based on these sequence data, full-length cDNA clones of BP-53 have been isolated, and the complete deduced sequence of BP-53 determined. This sequence contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids containing 18 cysteine residues clustered near the N- and C-terminus. The deduced protein sequence of BP-53 has 33% amino acid identity including conservation of all 18 cysteine residues with the recently cloned BP-28, a smaller human IGF-binding protein identified in amniotic fluid and also secreted by the cell line HEP G2. Expression of the cloned BP-53 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. This expressed protein is identical to plasma-derived BP-53 in its immunoreactivity, high affinity binding of IGF-I and IGF-II, and mobility on sodium dodecyl sulfate gel electrophoresis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cloning, Molecular*
  • Gene Expression Regulation*
  • Growth Hormone / pharmacology
  • Growth Hormone / physiology*
  • Humans
  • Insulin-Like Growth Factor Binding Proteins
  • Molecular Sequence Data
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Receptors, Somatomedin

Substances

  • Carrier Proteins
  • Insulin-Like Growth Factor Binding Proteins
  • Receptors, Cell Surface
  • Receptors, Somatomedin
  • Growth Hormone