Sulforaphane inhibits the proliferation of the BIU87 bladder cancer cell line via IGFBP-3 elevation

Asian Pac J Cancer Prev. 2014;15(4):1517-20. doi: 10.7314/apjcp.2014.15.4.1517.

Abstract

Aim: To investigate effects of sulforaphane on the BIU87 cell line and underlying mechanisms involving IGFBP-3.

Methods: Both BIU87 and IGFBP-3-silenced BIU87 cells were treated with sulforaphane. Cell proliferation was detected by MTT assay. Cell cycle and apoptosis were determined via flow cytometry. Quantitative polymerase chain reaction and Western blotting were applied to analyze the expression of IGFBP-3 and NF-κB at both mRNA and protein levels.

Results: Sulforaphane (80 μM) treatment could inhibit cell proliferation, inducing apoptosis and cell cycle arrest at G2/M phase. All these effects could be antagonized by IGFBP-3 silencing. Furthermore, sulforaphane (80 μM) could down-regulate NF-κB expression while elevating that of IGFBP-3.

Conclusions: Sulforaphane could suppress the proliferation of BIU87 cells via enhancing IGFBP-3 expression, which negatively regulating the NF-κB signaling pathway.

MeSH terms

  • Anticarcinogenic Agents / pharmacology
  • Apoptosis
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Humans
  • Insulin-Like Growth Factor Binding Protein 3 / biosynthesis*
  • Insulin-Like Growth Factor Binding Protein 3 / genetics
  • Isothiocyanates / pharmacology*
  • M Phase Cell Cycle Checkpoints / drug effects
  • NF-kappa B / biosynthesis
  • NF-kappa B / genetics
  • RNA Interference
  • RNA, Messenger / biosynthesis
  • RNA, Small Interfering
  • Signal Transduction / drug effects
  • Urinary Bladder Neoplasms / drug therapy*

Substances

  • Anticarcinogenic Agents
  • IGFBP3 protein, human
  • Insulin-Like Growth Factor Binding Protein 3
  • Isothiocyanates
  • NF-kappa B
  • RNA, Messenger
  • RNA, Small Interfering
  • sulforafan