Objectives: To expand the anatomical investigations of the G-spot and to assess the G-spot's characteristic histological and immunohistochemical features.
Design: An observational study.
Setting: International multicentre.
Population: Eight consecutive fresh human female cadavers.
Methods: Anterior vaginal wall dissections were executed and G-spot microdissections were performed. All specimens were stained with haematoxylin and eosin (H&E). The tissues of two women were selected at random for immunohistochemical staining.
Main outcome measures: The primary outcome measure was to document the anatomy of the G-spot. The secondary outcome measures were to identify the histology of the G-spot and to determine whether histological samples stained with H&E are sufficient to identify the G-spot.
Results: The anatomical existence of the G-spot was identified in all women and was in a diagonal plane. In seven (87.5%) and one (12.5%) of the women the G-spot complex was found on the left or right side, respectively. The G-spot was intimately fused with vessels, creating a complex. A large tangled vein-like vascular structure resembled an arteriovenous malformation and there were a few smaller feeding arteries. A band-like structure protruded from the tail of the G-spot. The size of the G-spot varied. Histologically, the G-spot was determined as a neurovascular complex structure. The neural component contained abundant peripheral nerve bundles and a nerve ganglion. The vascular component comprised large vein-like vessels and smaller feeding arteries. Circular and longitudinal muscles covered the G-complex.
Conclusion: The anatomy of the G-spot complex was confirmed. The histology of the G-spot presents as neurovascular tissues with a nerve ganglion. H&E staining is sufficient for the identification of the G-spot complex.
Keywords: G-spot; G-spot anatomy; G-spot histology; G-spot immunohistochemistry; Gräfenberg's zone; female erectile body; female prostate; female sexual function; vaginal anatomy.
© 2014 Royal College of Obstetricians and Gynaecologists.