Verification of the anatomy and newly discovered histology of the G-spot complex

BJOG. 2014 Oct;121(11):1333-9. doi: 10.1111/1471-0528.12707. Epub 2014 Mar 19.


Objectives: To expand the anatomical investigations of the G-spot and to assess the G-spot's characteristic histological and immunohistochemical features.

Design: An observational study.

Setting: International multicentre.

Population: Eight consecutive fresh human female cadavers.

Methods: Anterior vaginal wall dissections were executed and G-spot microdissections were performed. All specimens were stained with haematoxylin and eosin (H&E). The tissues of two women were selected at random for immunohistochemical staining.

Main outcome measures: The primary outcome measure was to document the anatomy of the G-spot. The secondary outcome measures were to identify the histology of the G-spot and to determine whether histological samples stained with H&E are sufficient to identify the G-spot.

Results: The anatomical existence of the G-spot was identified in all women and was in a diagonal plane. In seven (87.5%) and one (12.5%) of the women the G-spot complex was found on the left or right side, respectively. The G-spot was intimately fused with vessels, creating a complex. A large tangled vein-like vascular structure resembled an arteriovenous malformation and there were a few smaller feeding arteries. A band-like structure protruded from the tail of the G-spot. The size of the G-spot varied. Histologically, the G-spot was determined as a neurovascular complex structure. The neural component contained abundant peripheral nerve bundles and a nerve ganglion. The vascular component comprised large vein-like vessels and smaller feeding arteries. Circular and longitudinal muscles covered the G-complex.

Conclusion: The anatomy of the G-spot complex was confirmed. The histology of the G-spot presents as neurovascular tissues with a nerve ganglion. H&E staining is sufficient for the identification of the G-spot complex.

Keywords: G-spot; G-spot anatomy; G-spot histology; G-spot immunohistochemistry; Gräfenberg's zone; female erectile body; female prostate; female sexual function; vaginal anatomy.

Publication types

  • Review

MeSH terms

  • Adult
  • Cadaver
  • Clitoris / anatomy & histology*
  • Clitoris / blood supply
  • Clitoris / innervation
  • Female
  • Humans
  • Immunohistochemistry
  • Nerve Fibers / physiology*
  • Orgasm / physiology*
  • Peripheral Nervous System / physiology
  • Vagina / anatomy & histology*
  • Vagina / blood supply
  • Vagina / innervation