Characterization of the 17 Strains of Regulatory T Cell-Inducing Human-Derived Clostridia

Gut Microbes. May-Jun 2014;5(3):333-9. doi: 10.4161/gmic.28572. Epub 2014 Mar 18.

Abstract

The gut microbiota plays important roles in the development of the host immune system. We have previously shown that a combination of 46 strains of commensal Clostridia isolated from conventionally reared mice can induce the accumulation of CD4(+)Foxp3(+) regulatory T (Treg) cells in the mouse colonic lamina propria. Subsequently, we succeeded in isolating and selecting 17 strains of Clostridia from a healthy human fecal sample that can significantly increase the number and function of colonic Treg cells in colonized rodents, thereby attenuating symptoms of experimental allergic diarrhea and colitis. Here we characterize each of the 17 strains of human-derived Clostridia in terms of sensitivity to antibiotics and ability to produce short chain fatty acids and other metabolites, and discuss their potential as biotherapeutics to correct dysbiosis and treat immune-inflammatory diseases.

Keywords: Clostridia; Treg; gut microbiota.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Clostridium / drug effects
  • Clostridium / immunology*
  • Clostridium / isolation & purification
  • Clostridium / physiology*
  • Colon / microbiology
  • Cytosol / chemistry
  • Dysbiosis / therapy
  • Fatty Acids / analysis
  • Fatty Acids, Volatile / metabolism
  • Feces / microbiology
  • Healthy Volunteers
  • Humans
  • Mice
  • Microbial Sensitivity Tests
  • Probiotics / administration & dosage
  • Symbiosis*
  • T-Lymphocytes, Regulatory / immunology*

Substances

  • Anti-Bacterial Agents
  • Fatty Acids
  • Fatty Acids, Volatile